Device

Part:BBa_K1300007

Designed by: StuyGem   Group: iGEM14_StuyGem_NYC   (2014-06-19)

recA promoter + translational ccdB

The recA promoter is an inducible promoter that responds to DNA damage which can be caused by UV radiation (namely the formation of ssDNA). This promoter controls the expression of the ccdB gene. ccdB is a toxin that inhibits the function of DNA gyrase and interferes with helicase activity. As a result, processes such as DNA replication and RNA transcription are hindered, ultimately causing cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 430


[edit]
Categories
Parameters
None